Neoplasma Vol.53, p.9-14, 2006
|
| Title: Prognostic significance of tyrosinase mRNA detected by nested RT-PCR in patients with malignant melanoma
|
| Author: N., GLUMAC
; M., SNOJ
; M., HOCEVAR
; S., NOVAKOVIC
; |
|
Abstract: The aim of this study was to evaluate the role of tyrosinase
mRNAappearance in blood of malignant melanoma (MM) patients,
especially with advanced stages, for predicting the disease
progression, and consequently the survival. The tyrosinase
mRNA was measured by nested RT-PCR in peripheral venous blood
samples obtained from 86 patients (53 male and 33 female)
with mainly stage III and IV MM. The data were analyzed using
standard methods for survival analysis and logistic
regression.
Tyrosinase was negative in the MM patients with the disease stage
I or II, positive tyrosinase was in 11/50 patients with
stage III and in 5/22 patients with stage IV. Systemic metastases
developed in 14/16 patients with positive tyrosinase and in
41/70 with negative tyrosinase. The 3-year survival was 8% and 28%
among the patients with positive and the patients with
negative tyrosinase, respectively. The log rank test showed
statistically significant better survival of tyrosinase negative
patients
when compared to tyrosinase positive patients (p=0.039).
Multivariate analysis using logistic regression indicated
tyrosinase to be a statistically significant prognostic factor for
the survival ofMMpatients after controlling for Breslow and
ulceration values (p=0.006).
Positive tyrosinase in peripheral venous blood is statistically
significant, and more importantly independent negative predictor of
survival.
|
|
| Keywords: malignant melanoma, tyrosinase, mRNA, nested RT-PCR
|
| Year: 2006, Volume: 53, Issue: |
Page From: 9, Page To: 14 |
|
|
|
|
|
 download file |
|
|
|
|
