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Neoplasma Vol.52, p.18-24, 2005 |
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Title: An antigen recognized on cells in apoptosis detected by monoclonal antibody 2E12* | ||
Author: G., STIBRIKOVA ; I., MARINOV ; P., STOCKBAUER ; | ||
Abstract: Monoclonal antibody 2E12 was prepared by immunization of mice with
cells of a chronic myeloid leukemia cell line MOLM-7. Human
hematopoietic cell lines JURKAT, HPB-ALL, RC2A and MOLM-7 were
induced to receptor mediated apoptosis by the treatment with anti-
Fas monoclonal antibody 7C11 and subsequently tested for
reactivity with 2E12 antibody in comparison to staining with
annexin V-FITC and PI in the two-color immunofluorescence and flow
cytometry. After 2, 5, 24, and 48 hours of induction, a gradual
increase of the percentage of 2E12 positive cells in all cell
lines was observed, which partially correlated with an increase of
annexin V-FITC binding with a delay of about 12 hours. In the two-
color fluorescence microscopy the 2E12 antibody positivity was
restricted to the annexin V positive cells, but their number was
lower. The binding of 2E12 did not induce apoptosis nor influenced
the binding of annexin V. We suppose that the antibody 2E12
detects an antigen expressed on a subpopulation of cells in death.
Therefore it can be useful as a new marker for further dissection
between living, apoptotic and necrotic cellular populations in
vitro.
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Keywords: antibody 2E12, cell lines, apoptosis, anti-Fas, annexin V | ||
Year: 2005, Volume: 52, Issue: | Page From: 18, Page To: 24 | |
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