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Neoplasma Vol.53, p.191-199, 2006 |
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Title: Garlic-derived organosulfides induce cytotoxicity, apoptosis, cell cycle arrest and oxidative stress in human colon carcinoma cell lines | ||
Author: J., JAKUBIKOVA ; J., SEDLAK ; | ||
Abstract: Organosulfur compounds (OSC) from garlic, especially allicin
(ALI), diallyl sulfide (DAS), diallyl disulfide (DADS),
and diallyl trisulfide (DATS) are recognized as a group of
potential chemopreventive agents. In this study, we examined the
effects of OSC on human Caco-2 and HT-29 colon carcinoma cell
lines. Apoptosis induction (Annexin-V-FITC/PI,
fluorescein diacetate/PI, sub-G1 fraction), modulation of DNA cell
cycle (G2/M arrest, phospho-H3 mitotic marker),
transmembrane mitochondrial potential (JC-1) and intracellular GSH
amount (monochlorobimane assay) were measured by
flow cytometry and fluorimetry. Our results showed that order of
OSC-induced cell death in Caco-2 and HT-29 cells increased
in the range as follows: ALI < DAS = DADS < DATS and ALI = DAS < DADS < DATS,
respectively. Both cell lines
used are relatively resistant to OSC induced cytotoxicity, because
compound concentrations required to obtain significant
effect are in high micromolar range. ALI was less toxic than
equimolar doses of other OSC tested with the exception of GSH
modulation and G2/M arrest in Caco-2 cells. DADS-treated HT-29
cells and both DATS-treated cell lines exhibit inverse
correlation between p-H3 positivity and compound concentration due
to higher apoptotic rate. These results show the correlation
of sulfur atoms number in OSC with their capacity in apoptosis
induction and support the role of redox-sensitive
“sulfhydryl switches” in maintaining intracellular redox milieu.
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Keywords: organosulfur compounds, apoptosis, mitotic arrest, redox, human colon cancer cell lines | ||
Year: 2006, Volume: 53, Issue: | Page From: 191, Page To: 199 | |
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