Neoplasma Vol.52, p.225-230, 2005
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| Title: Oridonin-induced apoptosis in leukemia K562 cells and its mechanism
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| Author: J.J., LIU
; R.W., HUANG
; D.J., LIN
; X.Y., WU
; J., PENG
; X.L., PAN
; Y.Q., SONG
; Q., LIN
; M., HOU
; D.N., WANG
; F., CHEN
; M.H., ZHANG
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Abstract: Oridonin, an extract from the Chinese herb Rabdosia rubescens, is
currently one of the most important traditional Chinese
herbal medicines. Recently oridonin has been reported to have anti-
tumor effects in a large variety of malignant diseases.
In this study, we investigated the apoptotic inducing effect of
oridonin in leukemia K562 cells and its mechanism.
Cell growth inhibition was measured using a microculture
tetrazolium assay, apoptosis was measured by flow cytometry
and electron microscopy as well as by DNA fragmentation analysis.
Telomerase activity was measured by TRAP-enzyme-
linked immunosorbent assay, and the expression of Bcl-2 and Bax
proteins was detected by western blot analysis. The
results showed that oridonin could inhibit the proliferation and
induce apoptosis on leukemia K562 cells remarkably.
Telomerase activity as well as Bcl-2 expression was down-
regulated, while Bax expression was up-regulated concurrently,
when apoptosis ocurred. We therefore conclude that oridonin
demonstrated anti-proliferative and apoptosis-inducing effects
on K562 cells in vitro, and that changes in bcl-2 and bax protein
levels as well as telomerase activity may play an important
role in its mechanism of action.
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| Keywords: oridonin, apoptosis, Bcl-2, Bax, telomerase
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| Year: 2005, Volume: 52, Issue: |
Page From: 225, Page To: 230 |
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