Home HOME Acta Virologica 2005 Acta Virologica Vol.49, p.59-64, 2005

Journal info


Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

Impact Factor = 1.82

Aims and Scope
Abstracted and Indexed

Select Journal







Webshop Cart

Your Cart is currently empty.

Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.

Acta Virologica Vol.49, p.59-64, 2005

Title: MOLECULAR CHARACTERIZATION OF INFECTIOUS BURSAL DISEASE VIRUS ISOLATES FROM NEPAL BASED ON HYPERVARIABLE REGION OF VP2 GENE
Author: K. SHARMA, M. HAIR-BEJO, A.R. OMAR, I. AINI

Abstract: Two Infectious bursal disease virus (IBDV) isolates, NP1SSH and NP2K were obtained from a severe infectious bursal disease (IBD) outbreak in Nepal in 2002. The hypervariable (HV) region of VP2 gene (1326 bp) of the isolates was generated by RT-PCR and sequenced. The obtained nucleotide sequences were compared with those of twenty other IBDV isolates/strains. Phylogenetic analysis based on this comparison revealed that NP1SSH and NP2K clustered with very virulent (vv) IBDV strains of serotype 1. In contrast, classical, Australian classical and attenuated strains of serotype 1 and avirulent IBDV strains of serotype 2 formed a different cluster. The deduced amino acid sequences of the two isolates showed a 98.3% identity with each other and 97.1% and 98.3% identities, respectively with very virulent IBDV (vvIBDV) isolates/strains. Three amino acids substitutions at positions 300 (E?A), 308 (I?F) and 334 (A?P) within the HV region were common for both the isolates. The amino acids substitutions at positions 27 (S?T), 28 (I?T), 31 (D?A), 36 (H?Y), 135 (E?G), 223 (G?S), 225 (V?I), 351 (L?I), 352 (V?E) and 399 (I?S) for NP1SSH and at position 438 (I?S) for NP2K were unique and differed from other IBDV isolates/strains. NP1SSH and NP2K showed highest similarity (97.8%) with the BD399 strain from Bangladesh as compared with other vvIBDV isolates/strains. We conclude that the NP1SSH and NP2K isolates of IBDV from Nepal represent vvIBDV of serotype 1.

Keywords: Infectious bursal disease virus; VP2 gene; nucleotides sequence; deduced amino acids sequence; phylogenetic analysis; Nepal
Year: 2005, Volume: 49, Issue: Page From: 59, Page To: 64



download file



© AEPress s.r.o
Copyright notice: For any permission to reproduce, archive or otherwise use the documents in the ELiS, please contact AEP.