Neoplasma Vol.53, p.128-135, 2006
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| Title: Expression of MDR proteins in breast cancer and its correlation
with some clinical and pathological parameters
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| Author: S., RYBAROVA
; I., HODOROVA
; M., HAJDUKOVA
; K., SCHMIDTOVA
; J., MOJZIS
; K., KAJO
; Z., KVIATKOVSKA
; L., PLANK
; M., BENICKY
; A., MIROSSAY
; E., BIROS
; N., BOBROV
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Abstract: The aim of this work was to determine the expression of the
multidrug resistance (MDR) proteins, namely MDR1
(P-glycoprotein), MRP1 (multidrug resistance-related protein) and
LRP (lung resistance-related protein), in 87 samples of
breast carcinoma. Detection of these proteins was provided by
using indirect enzymatic immunohistochemistry. Our findings
were compared with the other clinical and pathological parameters:
expression of Her2/neu, estrogen receptor status
(ER), progesteron receptor status (PR), histological grade and
regional lymph node status. For statistical analysis, non-
parametric
two sided Mann-Whitney-U test was used. Majority of breast
carcinoma specimens show positivity for these proteins.
The MDR1 and MRP1 signal was found in the cytoplasm of cancer
cells. The expression of LRP was detected in the
cytoplasm close to the nuclear membrane. The samples were positive
for MDR1 protein in 57%, for MRP1 in 84% and for
LRP in 79%. Comparing our results with other clinical and
pathological parameters, negative correlation between ER, PR
and MDR1 expressions and histological grading status was found. No
associations were observed between the MRP1 and
LRP proteins and histological grading, as well as between the
expression of three MDR proteins and the other clinicaly relevant
parameters.
In conclusion, high frequency of expression ofMDRproteins in
breast carcinoma cells suggests, that these proteins might
be an important factor of drug resistance in breast carcinoma.
Nevertheles, the negative correlation between the histological
grade of malignancy of tumor and the expression of ER, PR and MDR1
indicates possible influence of progressive tumor
cell dediferentiation. However, this finding has to be confirmed
in additional evaluations.
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| Keywords: MDR1 protein, MRP1 protein, LRP protein, breast cancer,
immunohistochemistry
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| Year: 2006, Volume: 53, Issue: |
Page From: 128, Page To: 135 |
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