Neoplasma Vol.52, p.255-259, 2005
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| Title: Low mutational rate of K-ras codon 12 in singular bronchoscopy
specimens in suspected lung cancer
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| Author: I., SPASOVA
; H., NOVOTNA
; J., VACHTENHEIM
; H., BARTOSOVA
; J., PATEK
; V., HOSEROVA
; P., ZATLOUKAL
; Z., KINKOR
; |
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Abstract: Mutations of the K-ras gene are found in a subset of non-small-
cell lung carcinomas (NSCLC). The aim of our study was
to determine the K-ras codon 12 mutation in the first, singular
bronchoscopy specimen in parallel with the cytological examination
for the diagnosis of lung cancer.
Samples were obtained by diagnostic bronchoscopy in 140 patients
with suspected lung tumors. The analysis of K-ras
mutations was carried out by a sensitive two-step mutation-
enriched polymerase chain reaction-restriction fragment length
polymorphism (PCR-RFLP) assay. This method has been confirmed
earlier to be positive for mutated tumor cells and negative
for normal lung parenchyma and bronchus.
Of the 140 patients with suspected cancer, 93 were diagnosed as
NSCLC by cytology or histology in either the same specimen
used for the detection of K-ras mutation or in later biopsies.
However, only four K-ras codon 12 mutations were detected
in the first bronchoscopic material: one in adenocarcinoma, two in
squamous cell tumors, and one mutation was found
in a patient with dysplasia which was diagnosed later as a
squamous cell carcinoma.
Our findings indicate that although the K-ras (codon 12) mutation
is a gene lesion infrequently detectable in a singular
specimen taken at the first bronchoscopy examination in cases of
clinically suspected lung cancer, the detection of this mutation
can help to confirm the cytological diagnosis of NSCLC or may be
even diagnostic in cytologically negative cases.
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| Keywords: lung carcinoma, K-ras, codon 12, bronchoscopy
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| Year: 2005, Volume: 52, Issue: |
Page From: 255, Page To: 259 |
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