Journal info
|
||
Select Journal
Journals
Bratislava Medical Journal Endocrine Regulations General Physiology and Biophysics Neoplasma 2024 Ahead of print 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 Acta Virologica Studia Psychologica Cardiology Letters Psychológia a patopsych. dieťaťa Kovove Materialy-Metallic Materials Slovenská hudbaWebshop Cart
Your Cart is currently empty.
Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.
Neoplasma Vol.53, p.440-443, 2006 |
||
Title: Loss of heterozygosity on chromosomes 2p, 3p, 18q21.3 and 11p15.5 as a poor prognostic factor in stage II and III (FIGO) cervical cancer treated by radiotherapy | ||
Author: L., KOZLOWSKI ; T., FILIPOWSKI ; M., RUCINSKA ; W., PEPINSKI ; J., JANICA ; M., SKAWRONSKA ; J., POZNANSKI ; M.Z., WOJTUKIEWICZ ; | ||
Abstract: Loss of heterozygosity (LOH) has been shown to be an important
prognostic factor in a variety of malignant neoplasm’s.
Cervical cancer develops as result of multiple genetic
alterations. The aim of this study was to analyze presence of LOH
in
cervical cancer and to identify the correlation between LOH and
survival and relapse-free survival time in patients treated
with radiotherapy. Studies were performed on tumor specimens and
venous blood from 20 patients with cervical cancer
(squamous cell carcinoma G2 and G3) in stage II and III (FIGO)
treated with radiotherapy. DNA was isolated using organic
extraction. Additional microcolumn purification was performed. The
fluorescent multiplex polymerase chain reaction
(PCR) was used to amplify 10 microsatellite loci included in
commercially available human identification kits.
Microsatellite marker BAT 26 was amplified in separate PCR
reactions. 75% cervical cancers manifested LOH. LOH in
BAT 26 analysis (chromosome 2) was present in all these specimens.
60% of the cases showed LOH at one or more of other
examined loci (mostly on 3p, 18q21.3, and 11p15.5). Eight of nine
cervical cancers in clinical stage III showed LOH. All
cases of G3 squamous cell carcinoma of the cervix manifested LOH
on 2p. Patients with LOH have worse prognosis for survival
and relapse-free survival compared to patients without LOH.
|
||
Keywords: cervical cancer, loss of heterozygosity | ||
Year: 2006, Volume: 53, Issue: | Page From: 440, Page To: 443 | |
|
download file |
|