Neoplasma Vol.52, p.115-118, 2005
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| Title: CYP19 gene expression and aromatase activity in endometrial cancer
tissue: importance of the type of the disease
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| Author: L., BERSTEIN
; T., ZIMARINA
; A., KOVALEVSKIJ
; S., MAXIMOV
; O., VOLKOV
; |
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Abstract: Aromatase (CYP19) activity in malignant endometrium presents local
mechanism with potential ability to support tumor growth. The data
on interrelation between activity of this enzyme and its mRNA
signal in endometrial cancer (EC) tissue are very scarce and
inconclusive. To correct this gap we studied aromatase activity
and gene expression totally in 19 samples of EC (17 of them – from
postmenopausal women) collected during surgery. Aromatase activity
was evaluated by tritium water release assay and CYP19 gene
expression – with rt-PCR. Additionally, all studied EC cases were
divided on the basis of case history and some characteristics of
host and tumor and in accordance with existing classification into
cases belonging to type I or II of the disease (correspondingly,
13 and 6 observations). Positive correlation between enzyme
activity and CYP19 mRNA signal (Rs=+0.63, p<0.05) was revealed in
the whole group of studied samples. Aromatase activity varied in
evaluated material between 1.45 fM/mg prot./hr and 11.49 fM/mg
prot./hr, and in type II cases it was higher (7.27±0.96 fM/mg
prot/hr) than in type I observations (4.96±0.66 fM/mg prot./hr,
p=0.066). CYP19 mRNA expression was not revealed in 6 cases and
all of them belonged to the type I of disease. Thus, although type
II of EC is frequently considered as hormone non-dependent,
increased ability of this tumor type to estrogen biosynthesis (on
CYP19 gene and protein level) may lead to reconsideration of such
conclusion and warrants further investigation.
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| Keywords: endometrial cancer, aromatase, activity, CYP19 mRNA
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| Year: 2005, Volume: 52, Issue: |
Page From: 115, Page To: 118 |
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