Neoplasma Vol.52, p.25-31, 2005
|
| Title: TNF-đ induced changes in cell membrane antigen expression on K-562
cells associated with increased lactate dehydrogenase (LDH) release
|
| Author: V., JURISIC
; N., KRAGULJAC
; G., KONJEVIC
; I., SPUZIC
; |
|
Abstract: TNF-đ is a pleiotropic cytokine, which induces death of sensitive
cells, whose effect depend on cell membrane receptor expression,
cell cycle phases, as well as on intracellular ratio of pro-
apoptotic and anti-apoptotic molecule expression. Since
determination of LDH release from cultured cells in vitro,
reflects early membrane alterations, we estimated and compared LDH
release from cultured cells with changes in cell membrane antigen
expression on K-562 cells after TNF-đ treatment by flow cytometry.
The significant increase in LDH release activity and cytotoxicity
values was associated with decrease in membrane molecule
expression for CD45 and CD30 as well as for low expressed CD45RA
and CD38 after TNF-đ treatment. However, percentage of decrease of
all examined molecules is not uniform, and appears to depend on
the respective level of pre treatment values expression and
molecule type. These results indicated the complexity of events on
cell membrane, including association between increasing LDH
release and decrease of antigen expression of membrane molecules
following TNF-đ mediated processes.
|
|
| Keywords: antigen expression, apoptosisCD30, CD38, CD45, CD45RA, flow
cytometry, Lactate dehydrohenase (LDH) release assay, necrosis,
TNF-đ
|
| Year: 2005, Volume: 52, Issue: |
Page From: 25, Page To: 31 |
|
|
|
|
|
 download file |
|
|
|
|
