Neoplasma Vol.53, p.1-8, 2006
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| Title: Suppression of glucosylceramide synthase by RNA interference reverses multidrug resistance in human breast cancer cells
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| Author: Y.-L., SUN
; G.-Y., ZHOU
; K.-N., LI
; P., GAO
; Q.-H., ZHANG
; J.-H., ZHEN
; Y.-H., BAI
; X.-F., ZHANG
; |
|
Abstract: Glucosylceramide synthase (GCS), the enzyme that converts ceramide
to glucosylceramide, induce multidrug resistance
(MDR) in cancer cells. Recently, RNA interference (RNAi) is a
powerful strategy for gene therapy by introducing double-stranded RNA and leading to the sequence-specific destruction. We
have designed two different short hairpin RNAs
(shRNAs) targeting GCS and introduced them into adriamycin-
resistant human breast cancer cells (MCF-7/AdrR cells) to inhibit
GCS expression. The results demonstrated that the shRNAs targeting
GCS decreased GCS mRNA, abolished GCS protein
levels and restored the sensitivity of MCF-7/AdrR cells to several
antineoplastic drugs. This study revealed that this approach
can reverse MDR effectively and it may be applicable to cancer
patients as a specific means to restore the sensitivity
to chemotherapy.
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| Keywords: glucosylceramide synthase, multidrug resistance, RNA interference,
breast cancer
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| Year: 2006, Volume: 53, Issue: |
Page From: 1, Page To: 8 |
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