Neoplasma Vol.53, p.301-304, 2006
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| Title: Low expression of costimulatory molecules and mRNA for cytokines are important mechanisms of immunosuppression in acute lymphoblastic leukemia in children?
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| Author: W., LUCZYNSKI
; A., STASIAK-BARMUTA
; E., ILENDO
; O., KOVALCHUK
; M., KRAWCZUK-RYBAK
; I., MALINOWSKA
; M., MITURA-LESIUK
; L., CHYCZEWSKI
; M., MATYSIAK
; J., KOWALCZYK
; R., JAWOROWSKI
; |
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Abstract: Mechanisms leading blasts of acute lymphoblastic leukemia to
escape from immune surveillance are still unknown. Only
few reports showed that ALL cells are inefficient antigen
presenting cells. The aim of the study was to assess expression of
critical costimulatory/adhesion molecules and mRNAfor main pro-
and anti-inflammatory cytokines in ALL cells. Children
with B-cell precursor ALL (n=20) were prospectively enrolled into
the study. Expression of costimulatory/adhesion molecules
(CD1a, CD11c, CD40, CD54, CD80, CD83, CD86, CD123, HLA class I and
II) was assessed by flow cytometry and
mRNA for cytokines (IFN-gamma, IL-10, IL-4, TGF-beta) – with real-time
PCR.
Results: 1) high expression was observed for HLA I and II class,
moderate for CD40, CD83, CD86 and low or no expression
for CD80, CD54, CD1a, CD11c and CD123; 2) we found expression of
mRNA for IFN-gamma, IL-10, IL-4 and TGF-beta in
blasts cells (but not in all specimens). We noted relatively lower
expression of all assessed cytokines comparing to T-cells
obtained from healthy donors but interestingly expression for IL-
10 was higher in normal B-cells than in blast cells, and
IFN-gamma and IL-4 were not found in normal B-cells.
In summary we suggest that ALL-blasts present low expression of
costimulatory/adhesion molecules and mRNA for
cytokines and this probably contribute to the absence of host T-
cells stimulation to immune response.
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| Keywords: acute lymphoblastic leukemia, children, cytokines, flow cytometry,
real-time PCR, costimulatory molecules
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| Year: 2006, Volume: 53, Issue: |
Page From: 301, Page To: 304 |
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