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Neoplasma Vol.53, p.424-427, 2006 |
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Title: Immunogold detection of CD3 and CD4 antigens in patients with Mycosis fungoides | ||
Author: A.A., GRZANKA ; W., PLACEK ; A., GRZANKA ; | ||
Abstract: In this study, we analyzed the distribution of CD3 and CD4
antigens at the ultrastructural level in tissue samples from
mycosis fungoides patients using double-immunogold labeling. We
observed clusters composed of CD3 and CD4 antigens
on the plasma membrane and intracellulary. There were also
clusters only of one type of the antigen and we could observe
more often CD4 than CD3. Labeling of CD3 and CD4 was not found in
control cells incubated with non-immune serum. In
conclusion, our ultrastructural studies not only visualized
pattern distribution and relationship between CD3 and CD4 antigens
but might also suggest that the type and form of distribution
provides new clues to their possible translocation in
mycosis fungoides cells.
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Keywords: double-gold labeling, ultrastructure, mycosis fungoides, CD3 and CD4 receptors | ||
Year: 2006, Volume: 53, Issue: | Page From: 424, Page To: 427 | |
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