Neoplasma Vol.53, p.363-371, 2006
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| Title: Assay design and optimization of mutant-enriched PCR based method for detection of K-ras gene mutations in pancreatic carcinoma. |
| Author: Ugorcakova J, Hlavaty T, Babal P, Janega P, Bukovska G, Halgasova N, Hlavata A, Timko J, Duris I. |
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Abstract: The aim of our work was to develop a fast, reliable and sensitive PCR method to detect K-ras mutations in various clinical samples. There is a need for an unimpeachable method for early diagnosis and/or screening of pancreatic cancer (PC). We optimized and subsequently analyzed four methods based on mutant-enriched PCR for the sensitivity, cost and time expense. Using the selected optimal method we examined codon 12 K- ras mutations in a study population of 59 patients with upper GIT malignancies. Reliability of the genotyping was confirmed by sequencing. By using the best of our modified mutant-enriched PCR methods we achieved sensitivity of 1:1x10(exp5). Further studies are necessary to determine the optimal biological material sampling in PC.
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| Keywords: K-ras mutation, codom 12, pancreatic cancer, early detection, mutant-enriched PCR |
| Year: 2006, Volume: 53, Issue: |
Page From: 363, Page To: 371 |
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