Home Neoplasma 2006 Neoplasma Vol.53, p.363-371, 2006

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Founded: 1954
ISSN 0028-2685
ISSN 1338-4317 (online)

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Neoplasma Vol.53, p.363-371, 2006

Title: Assay design and optimization of mutant-enriched PCR based method for detection of K-ras gene mutations in pancreatic carcinoma.
Author: Ugorcakova J, Hlavaty T, Babal P, Janega P, Bukovska G, Halgasova N, Hlavata A, Timko J, Duris I.

Abstract: The aim of our work was to develop a fast, reliable and sensitive PCR method to detect K-ras mutations in various clinical samples. There is a need for an unimpeachable method for early diagnosis and/or screening of pancreatic cancer (PC). We optimized and subsequently analyzed four methods based on mutant-enriched PCR for the sensitivity, cost and time expense. Using the selected optimal method we examined codon 12 K- ras mutations in a study population of 59 patients with upper GIT malignancies. Reliability of the genotyping was confirmed by sequencing. By using the best of our modified mutant-enriched PCR methods we achieved sensitivity of 1:1x10(exp5). Further studies are necessary to determine the optimal biological material sampling in PC.

Keywords: K-ras mutation, codom 12, pancreatic cancer, early detection, mutant-enriched PCR
Year: 2006, Volume: 53, Issue: Page From: 363, Page To: 371

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