Home CONTACT Neoplasma 2009 Neoplasma Vol.56, No.1, p.13-21, 2009

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Founded: 1954
ISSN 0028-2685
ISSN 1338-4317 (online)

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Neoplasma Vol.56, No.1, p.13-21, 2009

Title: The inhibitory effect of human embryonic germ cells on ovarian cancer
Author: X. Song, B. Kong, D. Li, Q. Yang

Abstract:

Cancer cells share numerous characteristics with pluripotent stem cells which develop normally, contributing to the tumor cell plasticity. So we investigated the effect of human embryonic germ cells (hEGCs) on SKOV3 cells. We examined the efficacy of hEGCs through coculturing SKOV3 with hEGCs, detecting tunel apoptosis and caspase-9 activity by immunocytochemistry, and measuring the expression of AKT by real-time PCR and western blot. Further a xenograft model in SCID mouse to examine the effect of hEGCs on SKOV3 in vivo was used. Our results showed that there was a 1.5-fold growth reduction for SKOV3 in the coculture group. HEGCs induced apoptosis via caspase-9 activation and AKT down-regulation in SKOV3. This tumor cell inhibition was demonstrated also in the used animal tumor model. Taken together, our observations demonstrated that the hEGCs could inhibit the growth of SKOV3 by inducing apoptosis by inhibiting AKT pathway.



Keywords: embryonic germ cells; ovarian cancer; apoptosis
Year: 2009, Volume: 56, Issue: 1 Page From: 13, Page To: 21
doi:10.4149/neo_2009_01_13
Price: 21.60 €






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