Acta Virologica Vol.53, No.1, p.35-41, 2009

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.53, No.1, p.35-41, 2009
Title: Immunogenic properties of recombinant ectodomain of Newcastle disease virus hemagglutinin-neuraminidase protein expressed in Escherichia coli
Author: S. K. Wong, W. S. Tan, A. R. Omar, C. S. Tan, K. Yusoff

Abstract: Hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) plays a vital role in the viral infectivity, host immunity, and disease diagnosis. A portion of the HN gene encoding the ectodomain (nt 142–1739) was cloned and expressed in Escherichia coli yielding an insoluble HN protein and a soluble NusA-HN protein containing N-utilization substance A (NusA) fusion component. Both recombinant proteins were purified and used for immunization of chickens. The recombinant HN protein induced higher antibody titers as compared to the recombinant NusA-HN protein. These antibodies were able to react in immunoblot analysis with the corresponding recombinant proteins as well as with the HN protein of NDV.

Keywords: Newcastle disease virus; hemagglutinin-neuraminidase; cloning; expression; immunogenicity

Year: 2009, Volume: 53, Issue: 1	Page From: 35, Page To: 41
doi:10.4149/av_2009_01_35
Price: 16.80 €

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