Acta Virologica Vol.53, No.1, p.53-56, 2009

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.53, No.1, p.53-56, 2009
Title: Differentiation of Plum pox virus isolates by single-strand conformation polymorphism and low-stringency single specific primer PCR analysis of HC-Pro genome region
Author: S. Gadiou, D. Safarova, M. Navratil

Abstract: Single-strand conformation polymorphism (SSCP) and low-stringency single specific primer (LSSP)-PCR were assessed for suitability and reliability in genotyping of Plum pox virus (PPV) isolates. Examined PPV isolates included 16 PPV-D, 12 PPV-M, and 14 PPV-Rec isolates collected in Czech Republic. The analysis was performed on the helper component protease (HC-Pro) region of the PPV genome. SSCP and LSSP-PCR allowed the differentiation of PPV strain, but SSCP was not able to distinguish isolates within the same strain. The individual genotyping of each PPV isolate was obtained by LSSP-PCR. Nevertheless, both SSCP and LSSP-PCR techniques are suitable for preliminary screening of genetic variability of plant RNA viruses.

Keywords: Plum pox virus; strains; isolates; single-strand conformation polymorphism; low-stringency single primer PCR; HC-Pro region

Year: 2009, Volume: 53, Issue: 1	Page From: 53, Page To: 56
doi:10.4149/av_2009_01_53
Price: 9.60 €

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