Home CONTACT Neoplasma 2009 Neoplasma Vol.56, No.3, p.208-214, 2009

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Founded: 1954
ISSN 0028-2685
ISSN 1338-4317 (online)

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Neoplasma Vol.56, No.3, p.208-214, 2009

Title: Administration of isothiocyanate (E-4IB) and cisplatin leads to altered signalling and lysosomal export in human ovarian carcinoma sensitive- and cisplatin-resistant cells
Author: J. DURAJ, L. HUNAKOVA, J. BODO, J. JAKUBIKOVA, J. CHOVANCOVA, J. SEDLAK

Abstract: The aim of this study was to compare the effect of a new synthetic isothiocyanate derivative, ethyl 4-isothiocyanatobutanoate (E-4IB) and cisplatin (CDDP) in CDDP-sensitive human ovarian carcinoma cell line (A2780) and its resistant subline (A2780/CP). In parental cells, in comparison to untreated cells, sequential administration of both compounds led to higher exosomal dye (LysoTracker Green DND-26) retention and to alterations of mitogen-activated protein kinases (MAPKs), JNK, ERK and p38, or Akt kinase accompanied by changes in several anti- and pro-apoptotic molecules and lysosomal protein LAMP-1, as detected by Western blotting. On the contrary, variant A2780/CP cells were resistant to CDDP- or to combined sensitizer (E-4IB)/inducer (CDDP)-related apoptosis induction and exerted minor changes in the levels of these molecules.

Keywords: ovarian carcinoma cells, isothiocyanate E-4IB, cisplatin (CDDP), cisplatin (CDDP) resistance, apoptosis, lysosomes/exosomes
Year: 2009, Volume: 56, Issue: 3 Page From: 208, Page To: 214
doi:10.4149/neo_2009_03_208
Price: 16.80 €






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