Home Acta Virologica 2009 Acta Virologica Vol.53, No.2, p.99-104, 2009

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

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Acta Virologica Vol.53, No.2, p.99-104, 2009

Title: Use of Tomato leaf curl virus (TYLCV) truncated Rep gene sequence to engineer TYLCV resistance in tomato plants
Author: H. Ben_Tamarzizt, S. Gharsallah_Chouchane, R. Lengliz, D. P. Maxwell, M. Marrakchi, H. Fakhfakh, F. Gorsane

Abstract: Tomato yellow leaf curl disease causes severe losses in tomato production throughout Mediterranean countries including Tunisia. In order to generate engineered resistance to this disease, an intron-hairpin RNA construct harboring a Tomato yellow leaf curl Sardinia virus (TYLCSV) truncated replication-associated protein (Rep) gene was used to transform genotype of tomato plants. Prepared transgenic plants were agro-inoculated with Tunisian infectious strain of TYLCSV and screened for the resistance to infection. The infected transgenic plants were divided into 3 different groups according to their specific symptoms. Only one of them contained transgenic plants fully resistant to the tomato yellow leaf curl disease.

Keywords: Tomato yellow leaf curl virus; transgenic plants; resistance; Rep gene
Year: 2009, Volume: 53, Issue: 2 Page From: 99, Page To: 104
doi:10.4149/av_2009_02_99
Price: 14.40 €






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