Home Acta Virologica 2009 Acta Virologica Vol.53, No.2, p.121-124, 2009

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Quarterly, 72-88 pp. per issue
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

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Acta Virologica Vol.53, No.2, p.121-124, 2009

Title: Genotyping of hantaviruses occurring in Linyi, China, by nested RT-PCR combined with single-strand conformation polymorphism analysis
Author: J. Li, Y. X. Liu, Z. T. Zhao

Abstract: The hemorrhagic fever with renal syndrome (HFRS) incidence rate still holds in high level in some areas of the world, but a reliable and simple typing method that can be used in clinical diagnosis and epidemiologic surveys is not available. In this study, 48 serum samples were collected from patients with HFRS in Linyi area, China, that is seriously affected by this disease. The collected samples were analyzed by nested RT-PCR combined with single-strand conformation polymorphism (SSCP) for genotyping of hantaviruses (HV). Out of 48 serum samples, 41 samples were positive by the nested RT-PCR. According to the SSCP patterns, Seoul virus (SEOV) was found in 33 samples and Hantaan virus (HTNV) in 8 samples. The comparison of sequence identities of nested RT-PCR products of tested samples with reference isolates SEOV and HTNV supported the typing results. Thus, genotyping of HV by nested RT-PCR/SSCP is suitable in early diagnosis of HV infection and in epidemiologic surveys.

Keywords: hantaviruses; genotyping; nested RT-PCR; single-strand conformation polymorphism
Year: 2009, Volume: 53, Issue: 2 Page From: 121, Page To: 124
doi:10.4149/av_2009_02_121


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