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HOME General Physiology and Biophysics 2009 General Physiology and Biophysics Vol.28, No.2, p.146–159, 2009
HOME General Physiology and Biophysics 2009 General Physiology and Biophysics Vol.28, No.2, p.146–159, 2009
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General Physiology and Biophysics Vol.28, No.2, p.146–159, 2009 |
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| Title: Interaction of short-fragmented DNA with dipalmitoylphosphatidylcholine bilayers in presence of zinc | ||
| Author: Daniela Uhrikova, Petra Pullmannova, Margarida Bastos, Sergio S. Funari, Jose Teixeira | ||
| Abstract: The structure and temperature behaviour of the DNA+dipalmitoylphosphatidylcholine (DPPC) bilayer as a function of ZnCl2 concentration were examined using differential scanning calorimetry (DSC), small-angle neutron scattering (SANS) and small-angle X-ray diffraction (SAXD). Experiments revealed the coexistence of two lamellar phases in the mixture: the LPC phase, formed due to Zn2+ binding to the DPPC bilayers, and the condensed lamellar phase LDNA+PC with DNA strands packed between the DPPC bilayers. With increasing concentration of zinc, the temperature of the gel – liquid-crystal phase transition of DPPC increases in both phases, and the volume fraction of LDNA+PC phase decreases. In the gel state (at 20°C), the repeat distance of LDNA+PC phase is constant, dDNA+PC ~ 8.3 nm, up to 20 mmol/l of ZnCl2, and increases for higher concentrations of the salt. The periodicity of the LPC lamellar phase decreases substantially with the increasing concentration of the salt in the mixture. In the liquid-crystalline state, concentrations above 20 mmol/l ZnCl2 promote the dissolution of the LDNA+PC phase into DPPC + Zn2+ unilamellar vesicles and DNA is neutralized by Zn2+ ions. The screening of Zn2+ charge and the formation of a diffuse double layer due to increasing ionic strength of solution are responsible for the observed changes. |
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| Keywords: DPPC — DNA — Zinc — DSC — Small-angle scattering | ||
| Year: 2009, Volume: 28, Issue: 2 | Page From: 146, Page To: 159 | |
| doi:10.4149/gpb_2009_02_146 |
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