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Neoplasma Vol.56, No.5, p.371-378, 2009
|Title: A side population of cells from a human pancreatic carcinoma cell line harbors cancer stem cell characteristics|
|Author: Y. H. WANG, F. LI, B. LUO, X. H. WANG, H. C. SUN, S. LIU, Y. Q. CUI, X. X. XU|
|Abstract: We used flow cytometry and a DNA-binding dye efflux assay to isolate a side population (SP) of cells with stem cell characteristics from the human pancreatic carcinoma cell line, PANC-1. Non-obese diabetic/severe combined immunodeficiency mouse xenograft experiments showed that SP cells were enriched in tumor initiating capability compared with non-SP cells. Cultured SP cells were able to differentiate into daughter cells and non-SP cells, through asymmetric division. Our study demonstrated that SP cells had high drug-resistance, both in vivo and in vitro. SP cells also showed significantly higher levels of mRNA expression for CD133, ABCG2 and Notch1, when compared to non-SP cells. Furthermore, xenografted tumors derived from injected SP cells and treated with gemcitabine had more CD133+ cells than untreated ones. We therefore suggest that these SP cells from the PANC-1 cell line were enriched with cancer stem cells.
|Keywords: Cancer stem cell, PANC1 cell, side population (SP), CD133, ABCG2|
|Year: 2009, Volume: 56, Issue: 5||Page From: 371, Page To: 378|