Home FOR AUTHORS Neoplasma 2009 Neoplasma Vol.56, No.6, p.542-547, 2009

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ISSN 0028-2685
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Neoplasma Vol.56, No.6, p.542-547, 2009

Title: Genotoxic damage of human adipose-tissue derived mesenchymal stem cells triggers their terminal differentiation
Author: V. ALTANEROVA, E. HORVATHOVA, M. MATUSKOVA, L. KUCEROVA, C. ALTANER

Abstract: Human adipose tissue-derived mesenchymal (stromal) stem cells (AT-MSCs) and genetically modified to express cytosine deaminase:uracil phosphoribosyltransferase (CDy-AT-MSCs) were treated with hydrogen peroxide in order to induce DNA damage and subsequently evaluate their genetic stability by single cell gel electrophoresis. Both cells types (parental and transgene modified) did not differ in the sensitivity to DNA breaks induction. Potential tumorigenicity of AT-MSCs and CDy-AT-MSCs was tested by subcutaneous inoculation of cell suspension into flank of immunocompromised mice. Dose of 15x106 cells was not found to be tumorigenic in given experimental setup. AT-MSCs, CDy-AT-MSCs and MSCs isolated from human lipoma were treated with chemical carcinogen 4-nitroquinoline-1-oxide (4NQO) in attempts to transform them. Surviving cells after genotoxic stress were not transformed but underwent replicative senescence. Irreparable DNA damage caused triggered adipogenic terminal differentiation, rather than apoptosis induction in all kinds of cells tested.

Keywords: human adipose tissue-derived mesenchymal stem cells; genotoxic damage, single cell gel electrohoresis; chemical carcinogen 4NQO, terminal differentiation
Year: 2009, Volume: 56, Issue: 6 Page From: 542, Page To: 547
doi:10.4149/neo_2009_06_542
Price: 14.40 €






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