Home Acta Virologica 2009 Acta Virologica Vol.53, No.3, p.197-202, 2009

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Quarterly, 72-88 pp. per issue
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

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Acta Virologica Vol.53, No.3, p.197-202, 2009

Title: Genomic sequencing and characterization of a Chinese isolate of Bovine viral diarrhea virus 2
Author: L. Q. Zhu, Y. Q. Lin, X. Y. Ding, M. Ren, J. Tao, J. Y. Wang, G. P. Zhang, G. Q. Zhu

Abstract: The complete genomic sequencing and characterization of Bovine viral diarrhea virus (BVDV) isolate XJ-04 originated from cattle in China was described. The genomic RNA of the isolate was 12,284 nt long and contained short 5'- untranslated region (UTR), 3'-non-coding regions (NCR), and one open reading frame (ORF) encoding a large polyprotein of 3,895 amino acids with 20 potential N-glycosylation sites. The identity of the isolate XJ-04 with reference strains NADL (BVDV-1) and 890 (BVDV-2) in autoprotease (Npro) gene and structural genes (C, Erns, E1, E2) was analyzed. The percentage of nt and aa identity in analyzed genes indicated that the isolate XJ-04 was closer to the reference strain 890 (BVDV-2) than to NADL (BVDV-1). Phylogenetic analysis revealed that the isolate belonged to BVDV-2a subtype. Furthermore, comparison analysis indicated that the isolate XJ-04 did not contain any genomic insertions that can be directly related to the cytopathic phenotype.

Keywords: BVDV-2; cytopathic effect; genomic sequencing; phylogenetic analysis;
Year: 2009, Volume: 53, Issue: 3 Page From: 197, Page To: 202
doi:10.4149/av_2009_03_197
Price: 10.80 €






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