Acta Virologica Vol.53, No.3, p.203-206, 2009

Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

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Acta Virologica Vol.53, No.3, p.203-206, 2009
Title: Inhibition of Bovine herpesvirus multiplication in MDBK cells by small interfering RNAs
Author: P. S. Narute, A. A. Raut, M. Saini, A. Rai, P. K. Gupta

Abstract: Small interfering RNA (siRNA) mediated gene silencing is a promising approach in antiviral therapy. To investigate the antiviral effects of siRNAs on Bovine herpesvirus 1 (BoHV-1) multiplication, we designed and in vitro synthesized two siRNAs (siRNA-1 and siRNA-2) targeting the UL25 gene that is essential for BHV-1 multiplication. siRNA-1 and siRNA-2 inhibited the BoHV-1 multiplication in MDBK cells to a different extent, namely by 11% and 40%, respectively, as demonstrated by virus titers (TCID50/ml) determined in cell culture. This indicates that, in general, siRNAs can inhibit BHV-1 multiplication in vitro and could be used also against a BHV-1 infection in vivo following optimization of their application.

Keywords: siRNAs; Bovine herpesvirus 1; UL25 gene

Year: 2009, Volume: 53, Issue: 3	Page From: 203, Page To: 206
doi:10.4149/av_2009_03_203
Price: 9.60 €

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