Home Acta Virologica 2009 Acta Virologica Vol.53, No.4, p.247-253, 2009

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.53, No.4, p.247-253, 2009

Title: Characterization of ORF127 of Helicoverpa armigera nucleopolyhedrovirus
Author: Z. J. Guo, L. H. Qiu, M. F. Du, S. H. An

Abstract: It has been shown that Ha127 in the genome of Helicoverpa armigera nucleopolyhedrovirus (HaNPV) has homologs in some other baculoviruses and encodes a putative protein of 192 aa. In this study, a sequence analysis showed the transcription initiation site in Ha127 gene at nts 188 upstream of the translation initiation codon ATG and a potential leucine zipper motif at aa 34–55 in the corresponding protein. Ha127 transcripts were detected in HaNPV-infected HzAM1 cells at 18–72 hrs post infection ( p.i.) by RT-PCR, while the corresponding protein was found at 24–72 hrs p.i. by Western blot analysis suggesting that Ha127 is a late gene product. The size of detected Ha127 protein was about 28 K, a larger value than the predicted 22.6 K indicating a post-translational modification. Immunofluorescence assay of HzAM1 cells infected with HaNPV and Ha127-EGFP expression showed that Ha127 protein was localized in the nucleus. In summary, these data suggested that Ha127 was a functional ORF that might play a role in the nucleus during the late or very late gene expression.

Keywords: Helicoverpa armigera nucleopolyhedrovirus; ORF127; transcription; translation; sub-cellular localization
Year: 2009, Volume: 53, Issue: 4 Page From: 247, Page To: 253
doi:10.4149/av_2009_04_247


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