Home CUSTOMERS Acta Virologica 2009 Acta Virologica Vol.53, No.4, p.281-285, 2009

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

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Acta Virologica Vol.53, No.4, p.281-285, 2009

Title: Analysis of multiple virus-infected grapevine plant reveals persistence but uneven virus distribution
Author: P. Kominek, M. Glasa, M. Kominkova

Abstract: LN33 grapevine plants were artificially inoculated with budwoods originating from a field-cultivated Traminer grapevine which was naturally infected with Grapevine leafroll-associated virus 1 (GLRaV-1), Grapevine virus A (GVA), Grapevine virus B (GVB), Rupestris stem pitting-associated virus (RSPaV), and an unclassified tymovirus. Four years after inoculation, a comparison of the cane weights between healthy and infected grapevines did not show any significant difference. Corky bark symptoms or destructive effects of GVB infection never appeared on the infected grapevines. Dormant canes, sampled before the beginning of the vegetation period, were used for detection of grapevine viruses by ELISA or RT-PCR. ELISA turned out unexpectedly to be more effective than RT-PCR for detecting GLRaV-1 probably due to an insufficient specificity of the primers used, not reflecting the actual genetic variability of the virus. Distribution of viruses in the infected grapevines showed a different degree of irregularity in dependence on individual viruses. Therefore, in order to properly verify the sanitary status of grapevines under testing, several random samples from different parts of a tested plant have to be analyzed.

Keywords: grapevine; virus; corky bark; ELISA; RT-PCR
Year: 2009, Volume: 53, Issue: 4 Page From: 281, Page To: 285

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