Neoplasma Vol.57, No.4, p.299-305, 2010
|
| Title: Identification of differential proteins in colon cancer SW480 cells with HIF1-α silence by proteome analysis |
| Author: X. Y. Wu, Z. X. Fu, X. H. Wang, W. Shen |
|
Abstract: Hypoxia is one of the basic characteristics of the colorectal cancer and HIF1 plays a central role in tumor hypoxia adaptation and controls the expression of a variety of genes. But it had not reached a comprehensive understanding of them in colorectal cancer in hypoxia. This study silenced HIF1-α, which is the regulatory subunit of HIF1, and established two dimensional gel electrophoresis profiles for human SW480siHIF1-α and SW480N cells to identified proteins with differential expressions. Ten down-regulated proteins and nine up-regulated proteins were identified by HPLC-Chip-MS/MS. Down-regulated proteins including PLD2, ANXA2. HIF1-α had a clear positive correlation with PLD2 and ANXA2 in colon adenocarcinoma samples. There proteins might have a direct or indirect contact HIF1-α, and play a critical role in apoptosis, signal transduction and hypoxia adaptation.
|
|
| Keywords: colorectal neoplasms; hypoxia inducible factor1-α; RNA interference; proteomics analysis; phospholipase D2; annexin A2 |
|
|
|
| Year: 2010, Volume: 57, Issue: 4 |
Page From: 299, Page To: 305 |
doi:10.4149/neo_2010_04_299
|
|
 download file |
|
|
|
|
