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Acta Virologica Vol.54, No.2, p.99-104, 2010 |
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Title: Stable expression of Rice dwarf virus Pns10 suppresses the post-transcriptional gene silencing in transgenic Nicotiana benthamiana plants | ||
Author: P. Zhou, B. Ren, X. M. Zhang, Y. Wang, C. H. Wei, Y. Li | ||
Abstract: RNA silencing is a conserved mechanism that defends against viral infection and retrotransposon activity for protection of the genome. Segment 10 (S10) of Rice dwarf virus (RDV) encodes Pns10 protein, a viral suppressor of RNAi that suppresses the host RNA silencing machinery. In this study, we obtained stable transgenic RDV-S10 Nicotiana benthamiana plants expressing Pns10. Suppression of post-transcriptional gene silencing (PTGS) by Pns10 supported the conclusion that this protein exhibited the RNA silencing suppressor activity. In particular, the transgenic plants stably expressing a viral suppressor of RNAi (VSR) provide a model system for investigating the mechanism of RNA silencing. |
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Keywords: RNA silencing; VSR; Rice dwarf virus; Pns10; transgenic plant | ||
Year: 2010, Volume: 54, Issue: 2 | Page From: 99, Page To: 104 | |
doi:10.4149/av_2010_02_99 |
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