Home Acta Virologica 2010 Acta Virologica Vol.54, No.2, p.105-112, 2010

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

Impact Factor = 1.82

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Acta Virologica Vol.54, No.2, p.105-112, 2010

Title: Properties of Hepatitis C virus minigenome containing mutated 5'UTR region and luciferase transgene
Author: J. Yang, Y. F. Lei, Q. X. An, W. Yin, X. Lu, L. Ye, S. H. Wei, Z. S Jia, Z. K. Xu

Abstract: Sequences at the 3'UTR of Hepatitis C virus (HCV) negative-strand (-)RNA play an important role in the initiation of positive-strand (+)RNA synthesis. However, the underlying mechanism in cellular context is still unclear. In this report, we designed several cDNA-based HCV-like minigenomes containing different mutations at the 5'UTR of (+)RNA. These (+)RNAs transcribed from the minigenomes in vitro were transfected into HCV replicon cells for producing (-)RNAs with deletions of different stem loops (SL) at the 3'-end. The results showed that expression of the antisense transgene from minigenome increased, when the minigenome containing deletion of SL-C1+D1+E1 at the 3'-end of (-)RNA was transfected into the HCV replicon cells compared to that of the full minigenome. The expression of the transgene from minigenome decreased using other mutant minigenomes containing deletions SL-A1, SL-A1+B1, and SL-A1+B1+C1 at the 3'-end of (-)RNA. Finally, the transgene from SL-C1+D1+E1 of (-)RNA using CMV promoter-driven minigenome was expressed at higher level than full minigenome in HCV replicon cell lines. These results indicated that the region of (-)RNA interacting with HCV replicase may locate in the SL-C1+D1+E1 region of (-)RNA.

Keywords: Hepatitis C virus; minigenome; RNA dependent RNA polymerase; replication
Year: 2010, Volume: 54, Issue: 2 Page From: 105, Page To: 112
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