Acta Virologica Vol.54, No.2, p.131-136, 2010
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Title: HIV-1 Gag p24-Nef fusion peptide induces cellular and humoral immune response in a mouse model |
Author: M. Mahdavi, M. Ebtekar, K. Azadmanesh, H. R. Khorramkhorshid, F. Rahbarizadeh, M. H. Yazdi, R. Zabihollahi, M. Abolhassani, Z. M. Hassan |
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Abstract: Many Human immunodeficiency virus (HIV) candidate vaccines have been tested in clinical trials, but none was sufficiently effective in the prevention of HIV infection. A HIV vaccine should induce humoral as well as cell-mediated response, the latter including the cytotoxic CD8+ T lymphocyte (CTL) response. In this study, we immunized BALB/c mice with a purified fusion peptide Gag p24-Nef and evaluated immune responses. As for the cellular responses, the adjuvanted fusion peptide induced lymphocyte proliferation, CTL response, and cytokines IFN-γ and IL-4 in the Th1 pattern. Humoral immune response to the adjuvanted fusion peptide included an increase in IgG antibodies of more IgG2a than IgG1 subtype. These results indicate that the employed HIV-1 peptide construct can elicit both cellular and humoral immune responses in mice. Further studies aimed at memory T cells and other aspects of immune responses are needed before a comprehensive assessment of this candidate vaccine could be provided.
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Keywords: epitopes; fusion peptide; HIV-1 p24-Nef; immune response |
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Year: 2010, Volume: 54, Issue: 2 |
Page From: 131, Page To: 136 |
doi:10.4149/av_2010_02_131
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Price:
14.40 €
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