Acta Virologica Vol.54, No.3, p.165-172, 2010
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Title: Molecular characterization of long terminal repeat of porcine endogenous retroviruses in Chinese pigs |
Author: P. ZHANG, P. YU, W. WANG, L. ZHANG, S. F. LI, H. BU |
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Abstract: Pigs offer an unlimited source of xenografts for humans. However, vertically transmitted Porcine endogenous retrovirus (PERV) poses an infectious risk in the course of pig-to-human transplantation. In this study, we characterized PERV long terminal repeat (LTR) sequences from three species of Chinese pigs Banna minipig inbred (BMI), Wu-Zhi-Shan pig (WZSP), and Neijiang pig (NJP-A), and compared them with those of known PERVs (PERV-A, PERV-B, PERV-C, PERV-NIH, and 293-PERV-43). Genomic DNA extracted from peripheral blood mononuclear cells (PBMCs) of the Chinese pigs was used for PCR-amplification, cloning, and sequencing of LTRs. The sequences of BMI and WZSP LTRs were found identical with those of PERV-A and PERV-B, while that of NJP-A LTR was found close to those of PERV-C and PERV-NIH. The gammaretroviral nature of PERV LTRs from Chinese pigs was proved. These LTRs contained also promoter elements including enhancer-like repeats comparable with those of other PERVs. These findings suggested that PERVs from Chinese pigs were similar to PERV-A and PRV-B. Moreover, this study provided new data for the evaluation and selection of pigs to be used in the xenotransplantation.
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Keywords: xenotransplantation; Porcine endogenous retrovirus; long terminal repeat; sequence alignment; phylogenetic analysis |
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Year: 2010, Volume: 54, Issue: 3 |
Page From: 165, Page To: 172 |
doi:10.4149/av_2010_03_165
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Price:
19.20 €
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