Home Acta Virologica 2010 Acta Virologica Vol.54, No.3, p.173-180, 2010

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

Impact Factor = 1.82

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Acta Virologica Vol.54, No.3, p.173-180, 2010

Title: Characterization of antigens for Q fever serodiagnostics
Author: Z. Sekeyová, M. Kowalczewska, R. Vincentelli, P. Decloquement, G. Flores-Ramírez, Ľ. Škultéty, D. Raoult

Abstract: The aim of this study was to identify candidate proteins for serodiagnostics of Q fever by monoclonal antibodies (MAbs), and to clone, express, and purify the selected proteins for use as antigens in ELISA. The reactivity of three MAbs to Coxiella burnetii (C. b.) Nine Mile strain and one MAb to Priscilla strain was tested using SDS-PAGE, 2-D gel electrophoresis, immunoblot analysis, and mass spectrometry. Three immunoreactive Q fever-specific proteins discriminated by MAbs, namely the CBU_0937 protein, outer membrane Com1 (CBU_1910) protein, and elongation factor Tu (CBU_0236) were identified. Successful PCR-amplification, cloning, expression, and purification of the recombinant proteins Com1 and CBU_0937 allowed their use for the screening of sera from patients with Q fever endocarditis (18) or acute Q fever (16) in ELISA. The recombinant protein CBU_0937 with unknown biological function proved to be a more applicable diagnostic tool for Q fever ELISA as compared to the Com1 protein.

Keywords: Coxiella burnetii; monoclonal antibodies; 2-D electrophoresis; immunoblot analysis; recombinant protein; ELISA
Year: 2010, Volume: 54, Issue: 3 Page From: 173, Page To: 180
Price: 19.20 €

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