Acta Virologica Vol.54, No.3, p.221-224, 2010

Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

Impact Factor = 1.82

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Acta Virologica Vol.54, No.3, p.221-224, 2010
Title: Development of a probe-based blotting technique for the detection of Tobacco streak virus
Author: B. SAROVAR, D. V.R. SAIGOPAL

Abstract: Digoxigenin (DIG)-labeled DNA probe was developed for a sensitive and rapid detection of the Tobacco streak virus (TSV) isolates in India by dot-blot and tissue print hybridization techniques. DIG-labeled DNA probe complementary to the coat protein (CP) region of TSV sunflower isolate was designed and used to detect the TSV presence at field levels. Dot-blot hybridization was used to check a large number of TSV isolates with a single probe. In addition, a sensitivity of the technique was examined with the different sample extraction methods. Another technique, the tissue blot hybridization offered a simple, reliable procedure and did not require a sample processing. Thus, both non-radioactively labeled probe techniques could facilitate the sample screening during TSV outbreaks and offer an advantage in quarantine services.

Keywords: Tobacco streak virus isolates; DIG-labeled DNA probe; dot-blot hybridization; tissue print hybridization technique; diagnosis

Year: 2010, Volume: 54, Issue: 3	Page From: 221, Page To: 224
doi:10.4149/av_2010_03_221
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