Home Acta Virologica 2010 Acta Virologica Vol.54, No.3, p.225-228, 2010

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.54, No.3, p.225-228, 2010

Title: Molecular characterization of Ramie mosaic virus isolates detected in Jiangsu and Zhejiang provinces, China
Author: J. LI, X. Y. ZHANG, Y. J. QIAN

Abstract: Virus isolates were obtained from three ramie samples (Boehmeria nivea L.) showing yellow mosaic symptoms collected in Jiangsu and Zhejiang provinces, China. Comparison of partial DNA-A fragments amplified with begomovirus universal primers PA/PB revealed that these viral isolates shared a high sequence identity. The complete DNA-A sequences of two isolates J4 and Z1 were determined to be 2736 and 2737 nts, respectively, sharing 94.7% nucleotide sequence identity with each other. Also, the DNA-B components were identified for J4 and Z1 isolates and comprised 2717 and 2719 nts, respectively, sharing 88.6% nucleotide sequence identity with each other. Furthermore, sequence alignment and phylogenetic analysis showed that J4 and Z1 isolates had the highest sequence identities (93.6–94.7%) with isolates of Ramie mosaic virus (RamMV) for DNA-A. These molecular data suggested that J4 and Z1 may be two different isolates of RamMV. This is the first report about the occurrence of a bipartite begomovirus in these regions of China.

Keywords: begomovirus; ramie; bipartite; DNA-A; DNA-B
Year: 2010, Volume: 54, Issue: 3 Page From: 225, Page To: 228
doi:10.4149/av_2010_03_225


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