Home Neoplasma 2010 Neoplasma Vol.57, No.6, p.562-571, 2010

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Neoplasma Vol.57, No.6, p.562-571, 2010

Title: Heparanase participates in the growth and invasion of human U-2OS osteosarcoma cells and its close relationship with hypoxia-inducible factor-1α in osteosarcoma
Author: C. ZENG Z. KE, C. LUO, Z. YANG, L. WANG

Abstract:

Although the expression of heparanase is associated with invasion and metastasis of various human cancers, the effects of heparanase on human osteosarcoma have not been evaluated. We showed that down-regulating the expression of heparanase significantly reduced proliferation and invasion of human U-2OS osteosarcoma cells. Furthermore, heparanase silencing by short-hairpin RNA (shRNA) was associated with decreased hypoxia-inducible factor-1α (HIF-1α) level, implying that heparanase was associated with the expression of HIF-1α. This result was confirmed by immunohistochemistry analysis. In osteosarcoma tissues, immunohistochemical results revealed that heparanase expression had a close correlation with that of HIF-1α and they had a strong relation with presence of pulmonary metastasis (P<0.05).
Heparanase-positive samples had higher microvessel density (MVD) than heparanase-negative samples. Similarly, compared with HIF-1α-negative samples, HIF-1α-positive samples had higher MVD. Therefore, heparanase and HIF-1α facilitated tumor angiogenesis and promoted pulmonary metastasis of osteosarcoma.



Keywords: heparanase, HIF-1α, osteosarcoma, invasion, angiogenesis
Year: 2010, Volume: 57, Issue: 6 Page From: 562, Page To: 571
doi:10.4149/neo_2010_06_562
Price: 24.00 €






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