Home HOME Acta Virologica 2010 Acta Virologica Vol.54, No.4, p.281-285, 2010

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

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Acta Virologica Vol.54, No.4, p.281-285, 2010

Title: Evidence for the adaptive evolution of ORF5 gene of Porcine reproductive and respiratory syndrome virus isolated in China
Author: Z. XU, X. CHANG, S. XIAO, H. CHEN, R. ZHOU

Abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 gene encoding an envelope glycoprotein involved in humoral immunity is the most variable protein-coding gene of PRRSV. The present study aimed to identify potential selective pressures acting on the ORF5 gene of PRRSV isolates of North American type prevalent in China. The non-synonymous to synonymous rate ratio ω (dN/dS) was employed as a measure of selective pressure at the codon level. An overall ω of 0.45 indicated negative (purifying) selection as the major driving force operating on the ORF5 gene during adaptation of the virus to swine. Determination of ω values for individual amino acids sites revealed 8 positively selected sites, most of them situated in the N-terminal ectodomain, indicating their potential role in the binding of virus to the cellular receptors. Further, 75 negatively selected sites were identified in the rest of molecule, probably as a result of functional or immunological constraints. Determination of potential N-glycosylation sites revealed 7 sites, four of which coincided with the positively selected ones. These results indicated that a specific adaptive evolution has operated on the ORF5 gene of Chinese PRRSV isolates. It is hoped that the disclosed adaptive sites might help identify a candidate antigenic epitope for the use in vaccine against this serious swine disease.

Keywords: Porcine reproductive and respiratory syndrome virus; Chinese isolates; ORF5; adaptive evolution; positive selection; negative selection; glycosylation
Year: 2010, Volume: 54, Issue: 4 Page From: 281, Page To: 285
Price: 12.00 €

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