Home Acta Virologica 2010 Acta Virologica Vol.54, No.4, p.297-302, 2010

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.54, No.4, p.297-302, 2010

Title: Establishment of the cell line expressing human prion protein on PrP0/0 background
Author: M. PRČINA, P. FILIPČÍK, E. KONTSEKOVÁ

Abstract:  In this work we described preparation of the novel cell lines expressing human prion protein on PrP0/0-background. Prepared cell lines originated from the Nagasaki mice (PrP0/0) and showed a fibroblast phenotype. The expression level of human prion protein in the developed cell lines was comparable to the physiological expression measured in GT1-7 cells. A great advantage of the prepared cell lines was their short doubling time that allowed obtaining of a large amount of cells for the proteomic experiments. Newly established cell lines open a broad spectrum of applications in the prion research. Besides the study of physiological function of the prion protein or its interactome, the new cell lines could be successfully employed as a unique tool for the better understanding of key events in the pathogenesis of prion diseases.

Keywords: cell line; human prion protein; knockout; stable transfection
Year: 2010, Volume: 54, Issue: 4 Page From: 297, Page To: 302
doi:10.4149/av_2010_04_297


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