Neoplasma Vol.58, No.3, p.181-188, 2011
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| Title: Expression of soluble TGF-β receptor II by recombinant Vaccinia virus enhances E7 specific immunotherapy of HPV16 tumors |
| Author: K. ZURKOVA, P. CHLANDA, Z. SAMKOVA, K. BABIAROVA, L. KUTINOVA, J. KRYSTOFOVA, P. HAINZ, S. NEMECKOVA |
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Abstract: Therapeutic immunization with double recombinants of vaccinia virus (VACV) co-expressing sTβRII increased rejection of established TC-1 tumors in C57BL/6 mice in comparison with single recombinant expressing SigE7LAMP. Recombinant VACV derived from vaccination strain Praha expressed either the sTβRII (ectodomain) or chimeric protein fused to immunoglobulin Fc fragment (sTβRII-Fc-Jun) under control of two different promotors together with the immunogenic tumor associated antigen HPV16 E7 oncoprotein in a form of SigE7LAMP fusion molecule. The ability of soluble receptors to bind TGF-β in vitro was proved. Immunization of mice with double recombinant viruses and virus expressing SigE7LAMP only led to eliciting similar response of E7 specific CD8+ T cells as detected by IFN-γ ELISPOT.
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| Keywords: HPV16, E7; vaccinia virus; TGF beta; TGF beta receptor II; tumor; immunity |
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| Year: 2011, Volume: 58, Issue: 3 |
Page From: 181, Page To: 188 |
doi:10.4149/neo_2011_03_181
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