Home Acta Virologica 2011 Acta Virologica Vol.55, No.1, p.15-22, 2011

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Quarterly, 72-88 pp. per issue
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

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Acta Virologica Vol.55, No.1, p.15-22, 2011

Title: Development of single-cycle replicable human immunodeficiency virus 1 mutants
Author: R. ZABIHOLLAHI, S. M. SADAT, R. VAHABPOUR, M. R. AGHASADEGHI, A. MEMARNEJADIAN, T. GHAZANFARI, M. SALEHI, A. REZAEI, K. AZADMANESH

Abstract: Non-infectious but antigenic human immunodeficiency virus 1 (HIV-1) particles are essential tool for the research on many topics associated with this virus. Here we report the construction of plasmid containing the HIV-1 genome mutated in the pol gene, which was co-transfected with plasmids expressing the pol gene products reverse transcriptase (RT) and integrase (IN), and the glycoprotein G of vesicular stomatitis virus (VSV-G). The virions produced in HEK 293 T cells were antigenic, but able to replicate only for one cycle, e.g. first generation single-cycle replicable (SCR) virions. The presence of VSV-G in the envelope of these virions had to ensure a wider spectrum of susceptible cell types for the replication of SCR. Replication of the first generation SCR virions in HEK 293T, MT-2, and mouse spleen cells was examined by p24-capture ELISA, syncytium formation assay, and electron microscopy (EM). HEK 293T and MT-2 cell lines showed a similar replication capacity, while primary cultures of mouse spleen cells were much less effective. The infection of MT-2 cells with the first generation of SCR virions yielded the second generation SCR virions, which were non-infectious. Summing up, the HIV-1 SCR virions represent the useful tool for HIV-1 research facilitating a better biological safety. Moreover, considering their antigenic composition and limited replication, SCR virions may be a promising candidate for the vaccine studies.

Keywords: human immunodeficiency virus 1; mutants; single-cycle replicable virions; AIDS vaccine
Year: 2011, Volume: 55, Issue: 1 Page From: 15, Page To: 22
doi:10.4149/av_2011_01_15


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