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CUSTOMERS Acta Virologica 2011 Acta Virologica Vol.55, No.1, p.45-53, 2011
CUSTOMERS Acta Virologica 2011 Acta Virologica Vol.55, No.1, p.45-53, 2011
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Acta Virologica Vol.55, No.1, p.45-53, 2011 |
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| Title: N-terminal region of the PB1-F2 protein is responsible for increased expression of influenza A viral protein PB1 | ||
| Author: I. KOŠÍK, I. KREJNUSOVÁ, M. BYSTRICKÁ, K. POLÁKOVÁ, G. RUSS | ||
| Abstract: Influenza A virus (IAV) PB1-F2 protein is encoded by an alternative reading frame (+1) within the PB1 gene. PB1-F2 has been shown to contribute to the pathogenesis of influenza virus infection as well as to the secondary bacterial infection. More recently has been shown that PB1-F2 protein may regulate a viral RNA (vRNA) polymerase activity by the interaction with PB1 protein. We proved that PB1-F2 protein increased the level of expression of PB1 protein and vRNA in the infected cells. Moreover, we demonstrated that a higher level of vRNA expression resulted in the increase of expression of multiple viral proteins, including NP, M1, and NS1. Finally, we used plasmids expressing N-terminal (1–50 aa) or C-terminal (51–87 aa) region of the PB1-F2 molecule for transfection of MDCK cells co-infected with influenza A/Puerto Rico/8/34 (H1N1) virus deficient in the PB1-F2 protein expression (PR8ΔPB1-F2). These experiments clearly showed that N-terminal region of PB1-F2 protein was responsible for the increase in PB1 protein expression. C-terminal region of PB1-F2 protein had no effect. Thus, we have identified the important function for N-terminal region of PB1-F2 protein. |
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| Keywords: influenza virus; N-terminus; PB1-F2; PB1; vRNA polymerase | ||
| Year: 2011, Volume: 55, Issue: 1 | Page From: 45, Page To: 53 | |
| doi:10.4149/av_2011_01_45 |
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