Acta Virologica Vol.55, No.1, p.69-73, 2011

Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.55, No.1, p.69-73, 2011
Title: Antiserum to the recombinant truncated VP22 protein of herpes simplex virus type 1 that also recognizes full-length VP22
Author: M. L. LI, S. WANG, J. J. XING, CH. ZHENG

Abstract: The herpes simplex virus type 1 (HSV-1) tegument protein VP22 encoded by the UL49 gene is essential for HSV-1 infection. However, its precise functions in the virus life cycle are unknown. A relatively important tool for disclosing these functions is an antiserum specifically detecting VP22 in the infected cell. To this end, a recombinant truncated VP22 protein consisting of C-terminal 45 aa fused to EYFP (enhanced yellow fluorescent protein) and His-tag was expressed in Escherichia coli, purified by the Ni2+-NTA affinity chromatography, and used for the preparation of antiserum in rabbits. Western blot and immunofluorescence assay showed that this antiserum specifically detected purified truncated VP22 as well as full-length VP22 in the HSV-1 infected cells. These results indicate that the prepared antiserum could serve as a valuable tool for further studies of VP22 functions.

Keywords: herpes simplex virus type 1; truncated VP22; E. coli; recombinant protein; EYFP; antiserum

Year: 2011, Volume: 55, Issue: 1	Page From: 69, Page To: 73
doi:10.4149/av_2011_01_69
Price: 10.00 €

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