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Acta Virologica Vol.55, No.1, p.69-73, 2011 |
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Title: Antiserum to the recombinant truncated VP22 protein of herpes simplex virus type 1 that also recognizes full-length VP22 | ||
Author: M. L. LI, S. WANG, J. J. XING, CH. ZHENG | ||
Abstract: The herpes simplex virus type 1 (HSV-1) tegument protein VP22 encoded by the UL49 gene is essential for HSV-1 infection. However, its precise functions in the virus life cycle are unknown. A relatively important tool for disclosing these functions is an antiserum specifically detecting VP22 in the infected cell. To this end, a recombinant truncated VP22 protein consisting of C-terminal 45 aa fused to EYFP (enhanced yellow fluorescent protein) and His-tag was expressed in Escherichia coli, purified by the Ni2+-NTA affinity chromatography, and used for the preparation of antiserum in rabbits. Western blot and immunofluorescence assay showed that this antiserum specifically detected purified truncated VP22 as well as full-length VP22 in the HSV-1 infected cells. These results indicate that the prepared antiserum could serve as a valuable tool for further studies of VP22 functions. |
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Keywords: herpes simplex virus type 1; truncated VP22; E. coli; recombinant protein; EYFP; antiserum | ||
Year: 2011, Volume: 55, Issue: 1 | Page From: 69, Page To: 73 | |
doi:10.4149/av_2011_01_69 |
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