Home Acta Virologica 2011 Acta Virologica Vol.55, No.2, p.101-106, 2011

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ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.55, No.2, p.101-106, 2011

Title: Ubiquitins of Bombyx mori nucleopolyhedrovirus and Helicoverpa armigera nucleopolyhedrovirus show distinct subcellular localization in infected cells
Author: Z. J. GUO, Y. M. ZHU, G. H. LI, K. P. CHEN, C. X. ZHANG

Abstract: Ubiquitin (UB) is a conserved protein that regulates a number of processes in eukaryotic cells. Nearly all lepidopteran baculoviruses encode UB homologs showing a partial sequence identity with human UB (Hu-UB). In this study, the sequence, predicted 3D-structure and subcellular localization of UB homologs encoded by two different nucleopolyhedroviruses of Bombyx mori (BmNPV) and Helicoverpa armigera (HaNPV) were compared. UBs of BmNPV and HaNPV (Bm-UB, Ha-UB, respectively) shared only 73% of sequence identity of the different aa in relation to Hu-UB being localized in non-conserved parts, namely in two heterogeneous regions of aa 15–32 and aa 53–60. Interestingly, Bm-UB and Ha-UB share the same seven lysines except for an additional Lys54 in Bm-UB. However, in spite of the sequence heterogeneity, Bm-UB and Ha-UB have a similar predicted 3D-structure. A difference in their subcellular localization during virus growth in insect cell lines was found in the late stage of formation of occlusion-derived virus (ODV). In particular Bm-UB was localized mainly and evenly in the nucleus, while Ha-UB on the nuclear membrane. These data suggest that (i) UBs, besides being engaged in various cellular processes, have a role in specific processes of virus growth, and (ii) Bm-UB and Ha-UB may show certain different activities associated with the virus growth.

Keywords: : Bombyx mori nucleopolyhedrovirus ubiquitin; Helicoverpa armigera nucleopolyhedrovirus ubiquitin; sequence analysis; subcellular localization
Year: 2011, Volume: 55, Issue: 2 Page From: 101, Page To: 106
doi:10.4149/av_2011_02_101


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