Home Acta Virologica 2011 Acta Virologica Vol.55, No.2, p.169-174, 2011

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.55, No.2, p.169-174, 2011

Title: Minimal size of prototype foamy virus integrase for nuclear localization
Author: U. HYUN, D. H. LEE, C. G. SHIN

Abstract: We have reported previously that the prototype foamy virus (PFV) integrase (IN) has a strong nuclear localization signal (NLS) in its C-terminal domain, in particular in a region of aa 306–334 including highly karyophilic arginines or lysines at positions 308, 313, 318, 324, and 329. In this study, we used various mutants of the C-terminal domain to further analyze its karyophilic determinants. Plasmids expressing these mutants fused to maltose binding protein (MBP) and enhanced green fluorescent protein (EGFP) were transfected to COS-1 cells and subcellular localization of these fluorescent fusion proteins was determined by fluorescent microscopy. The results revealed that a maximum karyophilicity was exhibited by a region longer than the previously described one of 29 aa (aa 306–334), in particular by a 64 aa region (aa 289–352) with Arg341 and Lys349 as critical determinants.

Keywords: integrase; karyophilic determinants; nuclear localization; prototype foamy virus
Year: 2011, Volume: 55, Issue: 2 Page From: 169, Page To: 174
doi:10.4149/av_2011_02_169
Price: 12.00 €






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