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General Physiology and Biophysics Vol.30, Special Issue, p.54–60, 2011 |
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Title: Harmaline-resistant mutant of Methanothermobacter thermautotrophicus with a lesion in Na+/H+ antiport | ||
Author: Monika Vidová, Janette Bobáľová, Peter Šmigáň | ||
Abstract: A spontaneous mutant of Methanothermobacter thermautotrophicus resistant to the Na+/H+ antiporter inhibitor harmaline was isolated. The Na+/H+ exchange activity in the mutant cells was remarkably decreased in comparison with wild-type cells. Na+/H+ antiport activity of wild-type cells grown in the high Na+ concentration (125 mmol/l) was significantly increased as compared to the cells grown under low Na+ concentration (6.25 mmol/l) conditions. In contrast, harmaline resistant mutant showed almost the same Na+/H+ antiport activity under both these conditions. While harmaline profoundly inhibited methanogenesis in the wild-type, increased methanogenesis was observed both in the presence and absence of harmaline in the mutant strain. ATP synthesis driven by methanogenic electron transport was significantly enhanced in the mutant cells. The experimental data revealed the differential expression of A flavoprotein and molybdenum-containing formylmethanofuran dehydrogenase 1 subunit C in harmaline-resistant mutant. The overexpression of these proteins might contribute to harmaline resistance. Taken together the results indicate that harmaline resistance in this mutant has arisen as a consequence of mutation(s) in antiporter gene(s) or protein(s) linked to antiporter activity. Moreover this work provides the evidence that Na+/H+ exchanger deficiency in harmaline-resistant mutant can induce overexpression of several proteins participating in methanogenesis. |
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Keywords: Methanogens — Harmaline resistance — Sodium/proton exchange | ||
Year: 2011, Volume: 30, Issue: 5 | Page From: 54, Page To: 60 | |
doi:10.4149/gpb_2011_SI1_54 |
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