Home Neoplasma 2011 Neoplasma Vol.58, No.5, p.441-448, 2011

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Founded: 1954
ISSN 0028-2685
ISSN 1338-4317 (online)

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Neoplasma Vol.58, No.5, p.441-448, 2011

Title: Downregulation of fibulin-3 gene by promoter methylation in colorectal cancer predicts adverse prognosis
Author: J. D. TONG, N. L. JIAO, Y. X. WANG, Y. W. ZHANG, F. HAN

Abstract: Fibulin-3 gene has been identified as an antagonist of angiogenesis. We investigated the protein expression and promoter methylation status of fibulin-3 gene in colorectal cancer (CRC) and analyzed its correlation with clinicopathological factors. The study population enrolled 85 paired CRC specimens and adjacent normal tissues, as well as 32 cases of colorectal adenoma. Genomic DNA was extracted from paraffin-embedded samples using manual microdissection. Methylation-specific polymerase chain reaction (MSP) was used to determine the promoter methylation status and fibulin-3 gene expression was detected by immunohistochemistry. The results showed that, downregulation or silence of fibulin-3 protein was found in 57.6% (49/85) of CRC tissues, which was significantly higher than that of adjacent normal tissues (28.2%, 24/85) and colorectal adenoma (34.4%, 11/32) (P

Keywords: fibulin-3; methylation; immunohistochemistry; CRC; prognosis
Year: 2011, Volume: 58, Issue: 5 Page From: 441, Page To: 448
Price: 19.20 €

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