Home Endocrine Regulations 2011 Endocrine Regulations Vol.45, No.3, p.125-130, 2011

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Quarterly, 50 pp. per issue 
Founded: 1967
ISSN 1210-0668
E-ISSN 1336-0329

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Endocrine Regulations Vol.45, No.3, p.125-130, 2011

Title: The ability of hydroxylated estrogens (2-OH-E2 and 4-OH-E2) to increase of SHBG gene, protein expression and intracellular levels in MCF-7 cells line
Author: E. L. Gregoraszczuk, A. Ptak, A. Wrobel

Abstract: Objective. Sex Hormone-Binding Globulin (SHBG) – specific carrier for sex steroids – regulates hormone bioavailable fraction and estrogen signaling system in breast cancer cells. This  study was conducted to elucidate the effects of hydroxylated estrogen (E2) metabolites  (2-OH-E2 and 4-OH-E2) on sex hormone binding globulin (SHBG) mRNA and protein expression as well as on intracellular and extracellular SHBG levels.  
Methods. MCF-7 human breast cancer cells were cultured with 2-OH-E2 or 4-OH-E2 in concentration of 1, 10 and 100 nM for 24 h, 17β-estradiol being used as a positive control. SHBG levels were measured in medium and cells by ELISA, SHBG mRNA expression was evaluated by real-time-PCR and protein expression by Western blot analysis.
Results.  4-OH-E2 in high doses and 2-OH-E2 in the highest dose, while 17β-estradiol in all doses used increased intracellular but not extracellular SHBG levels. Both metabolites increased SHBG mRNA expression, the rank order of potency being E2 > 4-OH-E2 > 2-OH-E2. Both E2 and its metabolites increased SHBG protein expression.
Conclusion. Although the metabolites showed a lower potency than 17β-estradiol, further studies are needed to clarify whether hydroxylated metabolites of E2 are potent ligands for SHBG.

Keywords: 17β-estradiol, 2-OH-E2, 4-OH-E2, SHBG mRNA and protein expression, MCF-7
Year: 2011, Volume: 45, Issue: 3 Page From: 125, Page To: 130
Price: 12.00 €

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