Home FOR AUTHORS General Physiology and Biophysics 2011 General Physiology and Biophysics Vol.30, No.3, p.286–292, 2011

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Founded: 1982
ISSN  1338-4325 (online)

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General Physiology and Biophysics Vol.30, No.3, p.286–292, 2011

Title: Study of membrane attachment and in vivo co-localization of TerB protein from uropathogenic Escherichia coli KL53
Author: Olga Alekhina, Lenka Valkovicova, Jan Turna

Abstract:  The tellurite resistance operon has been found in a wide range of bacteria. We have previously identified the ter operon (terXYW and terZABCDEF) of the uropathogenic strain Escherichia coli KL53. In this study, we use an innovative approach to identify putative protein-protein interaction partners for one of the essential tellurite resistance proteins – TerB. We observe that N-terminus of TerB attaches to the periplasmic membrane, while the C-terminus is partly localized in the cytoplasm. Subsequently, by methods of in vivo cross-linking and mass-spectroscopic analysis, we have determined the proteins from both the membrane and cytoplasmic fractions, which can potentially interact with TerB.

Keywords: Tellurite resistance — Amphitropic protein — Cross-linking — TerB protein
Year: 2011, Volume: 30, Issue: 3 Page From: 286, Page To: 292

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